• Plant Pathology Department
• Wheat Page at KSU
• KSU Research & Extension
• GrainGenes
• Kansas Wheat Commission

Wheat Genetic and Genomic Resources Center
Kansas State University
Throckmorton Plant Sciences Center
Department of Plant Pathology
Manhattan, KS 66506-5502
785-532-2366 (Tel)
785-532-5692 (FAX)
wgrc@k-state.edu

IWGSC: INTEGRATED GENETIC AND PHYSICAL MAP OF CHROMOSOME 3A OF WHEAT

BAC Libraries

 

Library Code	Species	Variety	Chromosome	Genome fraction (%)	Number of Clones	Mean insert size	Coverage
TaaCsp3AShA	T. aestivum	Chinese Spring	3AS	2.1%	55,296	80 kb	10.9 x
TaaCsp3AShB	T. aestivum	Chinese Spring	3AS	2.1%	55,296	115 kb	15.9 x
TaaCsp3ALhA	T. aestivum	Chinese Spring	3AL	2.8%	55,296	106 kb	10.2 x

 

 

BAC LIBRARY DESCRIPTION

TaaCsp3AShA:

 

LIBRARY CODE	TaaCsp3AShA
SPECIES, CULTIVAR	Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY	3AS
DATE OF CONSTRUCTION	2006
PLACE OF ORIGIN	Olomouc, Czech Republic
CLONING SITE	HindIII
NUMBER OF CLONES	55,296
NUMBER OF PLATES	144 (No. 0001 - 0144)
NUMBER OF LIGATIONS	3
AVERAGE INSERT SIZE	80 kb
EMPTY CLONES	0.5%
PURITY OF THE SORTED FRANCTION	89%
GENOME/CHROMOSOME COVERAGE	10.9 x
VECTOR	pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE	Chloramphenicol (12.5 ug/ml)
HOST	Escherichia coli DH10B

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TaaCsp3AShB:

 

LIBRARY CODE	TaaCsp3AShB
SPECIES, CULTIVAR	Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY	3AS
DATE OF CONSTRUCTION	11/2008
PLACE OF ORIGIN	Olomouc, Czech Republic
CLONING SITE	HindIII
NUMBER OF CLONES	55,296
NUMBER OF PLATES	144 (No. 0145 - 0288)
NUMBER OF LIGATIONS	2
AVERAGE INSERT SIZE	115 kb
EMPTY CLONES	1.0%
PURITY OF THE SORTED FRANCTION	91%
GENOME/CHROMOSOME COVERAGE	15.9 x
VECTOR	pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE	Chloramphenicol (12.5 ug/ml)
HOST	Escherichia coli DH10B

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TaaCsp3ALhA:

 

LIBRARY CODE	TaaCsp3ALhA
SPECIES, CULTIVAR	Triticum aestivum cv. Chinese Spring
CHROMOSOME SPECIFICITY	3AL
DATE OF CONSTRUCTION	11/2007
PLACE OF ORIGIN	Olomouc, Czech Republic
CLONING SITE	HindIII
NUMBER OF CLONES	55,296
NUMBER OF PLATES	144 (No. 0001 - 0144)
NUMBER OF LIGATIONS	4
AVERAGE INSERT SIZE	106 kb
EMPTY CLONES	5.4%
PURITY OF THE SORTED FRANCTION	87%
GENOME/CHROMOSOME COVERAGE	10.2 x
VECTOR	pIndigoBAC-5 (Epicentre)
ANTIBIOTIC RESISTANCE	Chloramphenicol (12.5 ug/ml)
HOST	Escherichia coli DH10B

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