Herman Lab Protocols

Worm Protocols
  1. EMS mutagenesis
  2. MMS integration
  3. Nematode dye filling
  4. Double dye fill
  5. Worm PCR
  6. Genomic DNA prep
  7. In situ hybridization
  8. Yale RNA prep
  9. Joe's mRNA prep
  10. Fermentor
  11. Growing worms in liquid
Molecular Biology
  1. Cosmid prep
  2. Qiagen cosmid prep
  3. cDNA phage stock / in vivo excision
  4. Standard PCR
  5. Long PCR
  6. Labeling reaction
  7. Probe counter
  8. Library titer
  9. Library screen
  10. Sequencing reactions
  11. Sequencing procedure
  12. Competent cells
  13. Yeast transformation

Disclaimer: These protocols have been collected from labs of the C. elegans community as well as other sources. We have recognized, to the best of our knowledge, the sources of the protocols. If you recognize a protocol that is not properly attributed, please notify us and we will make the correction. Also note that most of the protocols have been modified for use in our lab and its inherent resources.