Optical Studies of Single Molecules and
Molecular Assemblies in Chemical Physics and Biophysics
ACS National
Meeting, San Diego, CA, April 1-5, 2001
Anne Myers
Kelley (Kansas State University) amkelley@ksu.edu, tel. 785-532-3843
Shimon
Weiss (Lawrence Berkeley National Laboratory) sweiss@lbl.gov,
tel. 510-486-5202
Until quite recently, essentially
all physical measurements on molecular-scale entities involved averaging over
large ensembles. The ability to interrogate
individual molecules and molecular assemblies on a one-by-one basis is driving
revolutionary changes in our understanding of fundamental chemical and
biological processes. This symposium
will focus on optical probes (fluorescence, absorption, Raman, and nonlinear
spectroscopies) of the chemical physics and biophysics of molecules and
supramolecular structures in which single-molecule techniques can reveal
important heterogeneities hidden in traditional ensemble measurements. Systems of interest include single molecules
in liquids and solids and on surfaces, metallic and semiconductor
nanoparticles, J-aggregates, conducting polymers, molecularly doped sol-gels,
single molecules in biomembranes, single peptides, enzymes, structural and
motor proteins, single oligonucleotides, DNAs, and RNAs, and single
photosynthetic reaction centers.
Supported by:
Becker & Hickl GmbH, Carl
Zeiss, Roper Scientific, PicoQuant
GmbH, Coherent, Inc.
Invited speakers:
• Sunney Xie (Dept. of Chemistry and
Chemical Biology, Harvard Univ., Cambridge, MA; xie@chemistry. harvard.edu) Single-Molecule
Enzymatic and Conformational Dynamics
• W.E. Moerner (Dept. of Chemistry, Stanford Univ.,
Stanford, CA; w.e.moerner@stanford.edu) “Single-molecule spectroscopy, from quantum optics to molecular motors”
• Robin
Hochstrasser (Dept. of Chemistry, Univ. of Pennsylvania, Philadelphia, PA;
hochstra@sas.upenn.edu) “Equilibrium fluctuations and energy transfer of reactions and
conformational dynamics of proteins”
• Kazuhiko Kinosita Jr.
(Department of Physics, Faculty of Science and Technology, Keio University,
Yokohama, Japan; kazuhiko@phys.keio.ac.jp) “Optical imaging of rotary molecular motors”
• Toshio Yanagida (Dept. of
Physiology and Biosignaling, Osaka University School of Medicine, Osaka, Japan;
yanagida@bpe.osaka-u.ac.jp) “Single
molecule biophysics”
• Thomas Schmidt (Leiden
Institute of Physics, Leiden University, Leiden, The Netherlands;
tschmidt@biophys.leidenuniv.nl) “Single-molecule
imaging for the study of biological membranes”
• Claus Seidel
(Max-Planck-Institut für Biophysikalische Chemie, Am Fassberg 11, D-37077
Göttingen, Germany; cseidel@gwdg.de) “Analysis of molecular structure and dynamics by selective
single-molecule fluorescence spectroscopy”
• Markus Sauer
(Physikalisch-Chemisches Institut, Universität Heidelberg, Heidelberg, Germany;
sauer@urz.uni-heidelberg.de) “Spectrally-resolved
fluorescence lifetime imaging of single molecules”
• Jan Schmidt (Huygens Laboratory,
Centre of the Study of Excited States of Molecules, Leiden University, Leiden,
The Netherlands; mat@molphys.leidenuniv.nl) “Single-molecule spectroscopic studies of light-harvesting complexes of
photosynthetic bacteria”
• Michel
Orrit (C.P.M.O.H., CNRS et Université Bordeaux I, Talence, France;
orrit@yak.cpmoh.u-bordeaux.fr) “Dynamics of solids at low temperatures
studied with single molecules”
• Paul Barbara
(Dept. of Chemistry, University of Texas, Austin TX; p.barbara@mail.utexas.edu)
“Single molecule spectroscopy of
conjugated polymers”
• Ed Yeung
(Department of Chemistry, Iowa State University, Ames, IA; yeung@ameslab.gov) “Single-molecule disease diagnosis based on
electrophoresis and spectroscopy”
• Christian G. Hübner representing
group of Urs Wild
(Laboratorium für Physikalische Chemie, ETH-Zentrum, Zürich, Switzerland;
wild@phys.chem.ethz.ch) “Direct
observation of the influence of oxygen on the singlet and triplet lifetime of
single dye molecules”
• Thomas Basché (Institut fuer Physikalische Chemie, Johannes Gutenberg-Universität, Mainz, Germany; thomas.basche@uni-mainz.de) “Confocal fluorescence and Raman spectroscopy of single nanoparticles”
• Katrin
Kneipp (Technical University of Berlin, Physics Department, Berlin,
Germany, and MIT; Kneipp@usa.net)
“Ultrasensitive Raman
spectroscopy at the single molecule level based on surface-enhanced Raman
scattering on silver and gold colloidal clusters”
• Bob Dunn (Dept. of Chemistry,
University of Kansas, Lawrence, KS; rdunn@caco3.chem.ukans.edu) “Single molecule studies of artificial
pulmonary lung surfactant”
• Dan
Higgins (Dept. of Chemistry, Kansas State University, Manhattan, KS;
higgins@ksu.edu) “Molecular scale environments in organic and inorganic
composite films probed by single molecule spectroscopy”
• Shuming Nie
(Department of Chemistry, Indiana University, Blooomington, IN;
nie@indiana.edu) “Probing single
molecules and single nanoparticles by surface-enhanced Raman scattering”
• Peter Wolynes (Department of
Chemistry, University of Illinois, Urbana, IL
61801; wolynes@scs.uiuc.edu) Intermittency of Activated Events in Single
Molecules
• Rob Dickson
(School of Chemistry and Biochemistry, Georgia Institute of Technology,
Atlanta, GA; dickson@chemistry.gatech.edu) “Three-dimensional single molecule orientational microscopy and
dynamics”
• Kurt Thorn representing group of
Ron Vale (Dept. of
Pharmacology, University of California, San Francisco, CA; vale@phy.ucsf.edu,
thorn@itsa.ucsf.edu)
“Deciphering kinesin motility
with single molecule microscopy”
• Norman Dovichi (Dept. of Chemistry,
University of Alberta, Edmonton, Alberta, Canada; norm.dovichi@ualberta.ca)
“Michaelis-Menten kinetics of a
single molecule of calf-intestinal alkaline phosphatase”
• Mikael Käll
(Dept. of Applied Physics, Chalmers University of Technology, Göteborg, Sweden;
kall@fy.chalmers.se) “Inhomogeneous broadening and single molecule fine-structure
in surface-enhanced Raman spectra of proteins and amino acids”
• Eitan Geva (Dept. of Chemistry, Univ. of Michigan, Ann Arbor, Michigan; eitan@umich.edu) “Single molecule spectroscopy in glasses”
• Louis Brus (Dept. of Chemistry, Columbia University, New York, NY; brus@chem.columbia.edu) “Raman spectra of single R6G molecules on Ag nanocrystal aggregates”
• Jörg Wrachtrup (Institute der Physik, Universität Stuttgart, Stuttgart, Germany; j.wrachtrup@physik.uni-stuttgart.de ) “Observation of charge and energy transfer in single photosynthetic reaction centers”
• Steven
Chu (Dept. of Physics, Stanford Univ., Stanford, CA; schu@stanford.edu)
“Single molecule fluorescence studies
of ribozymes and proteins”
8:20 — 1. Deciphering kinesin motility with single
molecule microscopy. K. S. Thorn, M. Tomishige, R. D. Vale
9:00 — 2. Optical imaging of rotary molecular motors. K. Kinosita Jr.
9:40 — 3. Observation of a single dye labeled virus on its infectious
entry pathway into a living cell. C. Bräuchle
10:00 — Intermission.
10:20 — 4. Single molecule biophysics. T. Yanagida
11:00 — 5. Single-molecule spectroscopy, from quantum optics to
molecular motors W. E. Moerner, B. Lounis, H. Sosa, E. J. G. Peterman,
L. S. B. Goldstein
11:40 — 6. Single-molecule dynamics and interactions at living
interfaces. X. N. Xu, R. Jeffers, S. Huang, D. Wang, M. Nowak
1:40 — 23. Michaelis-Menten kinetics of a single
molecule of calf-intestinal alkaline phosphatase. N. J. Dovichi, R.
Polakowski, M. Eggerston
2:20 — 24. Single-molecule enzymatic and conformational dynamics. X.
S. Xie
3:00 — 25. Conformational substates dynamics in the active adrenergic
receptor. G. Peleg, P. Ghanouni, B. K. Kobilka, R. N. Zare
3:20 — Intermission.
3:40 — 26. Spectrally-resolved fluorescence lifetime imaging of single
molecules. M. Sauer
4:20 — 27. New tools for single molecule dynamics: From trapping in
vesicles to surface enhanced Raman scattering. G. Haran, E. Boukobza, A.
Weiss
4:40 — 28. Simultaneous "dual-color and dual-polarization
imaging" as a tool for co-localization and fluorescence resonant energy
transfer studies of single molecules. G. Harms, L. Cognet, G. A. Blab,
P. H. M. Lommerse, T. Schmidt
5:00 — 29. Single-molecule spectroscopy study of the mechanisms and
dynamics of molecular interactions and enzymatic reactions. H. P. Lu, Y.
Chen
Monday morning (Chair, Anne Kelley)
8:20 — 49. Dynamics of solids at low temperatures
studied with single molecules. J. Caruge, M. Orrit
9:00 — 50. Single molecule spectroscopy in glasses. E. Geva, J. L.
Skinner
9:40 — 51. Fluctuation in single molecule spectroscopy. Y. Jung,
E. Barkai, R. J. Silbey
10:00 — Intermission.
10:20 — 52. Confocal fluorescence and Raman spectroscopy of single
nanoparticles. T. Basché, A. Mews
11:00 — 53. Spectroscopy of single Si nanoparticles. S. K. Buratto,
M. D. Mason, P. J. Carson, G. M. Credo
11:20 — 54. Photon antibunching in the fluorescence of single molecules
embedded in a thin polymer film. F. Treussart, C. Grossman, R. Pansu, J.
Roch
11:40 — 55. Non-Lorentzian spectral line shapes of single molecules:
Beyond the sudden jump model. T. Plakhotnik
1:40 — 80. Analysis of molecular structure and
dynamics by selective single-molecule fluorescence spectroscopy. C. A. M. Seidel,
E. Schweinberger, S. Berger, C. Eggeling, J. Schaffer, J. Widengren, S.
Felekyan
2:20 — 81. Single molecule fluorescence studies of ribozymes and
proteins. S. Chu
3:00 — 82. Single-molecule imaging and time-resolved fluorometry of an
analog of adenine. M. Ishikawa, J. Ye
3:20 — Intermission.
3:40 — 83. Single-molecule disease diagnosis based on electrophoresis
and spectroscopy. E. S. Yeung, M. R. Shortreed, Y. Ma, S. H. Kang, H. Li
4:20 — 84. Single-molecule imaging for the study of biological membranes.
T. Schmidt
5:00 — 85. Theoretical studies of room-temperature single molecule
experiments. J. Cao
8:20 — 109. Inhomogeneous broadening and single
molecule fine-structure in surface-enhanced Raman spectra of proteins and amino
acids. M. Käll, E. J. Bjerneld, H. Xu, P. Johansson
9:00 — 110. Probing single molecules and single nanoparticles by
surface-enhanced Raman scattering. S. Nie
9:40 — 111. Correlation of SERS activity and micro-structure of silver colloids.
Y. Maruyama, M. Ishikawa, M. Futamata
10:00 — Intermission.
10:20 — 112. Raman spectra of single R6G molecules on Ag nanocrystal
aggregates. L. Brus
11:00 — 113. Ultrasensitive Raman spectroscopy at the single molecule
level based on surface-enhanced Raman scattering on silver and gold colloidal
clusters. K. Kneipp, H. Kneipp, I. Itzkan, R. R. Dasari, M. S. Feld
11:40 — 114. Photoactivated fluorescence from individual silver
nanoclusters. L. A. Peyser, A. E. Vinson, A. P. Bartko, R. M. Dickson
8:20 — 143. Single molecule studies of artificial
pulmonary lung surfactant. B. N. Flanders, S. A. Vickery, R. C. Dunn
9:00 — 144. 3-D Single molecule orientational microscopy and dynamics. R.
M. Dickson
9:40 — 145. Probing surface diffusion heterogeneity at biomaterial
interfaces with single-event histograms. D. L. Burden, S. D. Poppen, J.
T. Elliott, J. T. Woodward, A. Sehgal
10:00 — Intermission.
10:20 — 146. Direct observation of the influence of oxygen on the
singlet and triplet lifetime of single dye molecules. C. G. Hübner, I.
Renge, A. Renn, U. P. Wild
11:00 — 147. Lateral diffusion of non-tethered phospholipids in
polymer-tethered phospholipid membranes studied at the single molecule level. C.
A. Naumann, P. C. Ke
11:20 — 148. Single molecule studies of heterogeneous dynamics near the
glass transition. L. A. Deschenes, D. A. Vanden Bout
11:40 — 149. Time resolved fluorescence spectroscopy on the
photo-physical behavior of single molecules and quantum dots. P. L. T. M.
Frederix, W. G. J. H. M. Van Sark, D. J. Van den Heuvel, E. L. De Beer, H.
C. Gerritsen
1:40 — 173. Molecular scale environments in organic
and inorganic composite films probed by single molecule spectroscopy. D. A.
Higgins, M. M. Collinson, A. M. Bardo, E. Mei, Y. Hou, R. Allenbaugh, S.
Ginagunta
2:20 — 174. Single molecule spectroscopy of conjugated polymers. P.
F. Barbara, D. Hu, J. Yu, N. W. Song
3:00 — 175. Single molecule probing of nanoscale polymer dynamics. M.
F. Garcia-Parajo, J. A. Veerman, R. Bouwhuis, R. Vallee, J. G. Vancso, N.
F. van Hulst
3:20 — Intermission.
3:40 — 176. Intermittency of activated events in single molecules. P.
Wolynes
4:20 — 177. Imaging the excitation profile of a confocal microscope for
correlated fluorescence/AFM of individual nanosized particles and biomolecules.
A. Van Orden, D. Willard, L. Kolodny, L. Carillo, M. Nelson
4:40 — 178. Probing single-ion luminescence in rare-earth doped
nanocrystals. M. D. Barnes, A. Mehta, T. Thundat, R. Bhargava
5:00 — 179. Excitons and polaritons in individual J-aggregate fibers and
organic-inorganic CdSe nanocrystal systems. M. Vacha, S. Takei, H.
Suzuki, K. Hashizume, M. Furuki, L. S. Pu, T. Tani
8:20 — 356. Observation of charge and energy transfer
in single photosynthetic reaction centers. J. Wrachtrup, F. Jelezko, C.
Tietz
9:00 — 357. Single-molecule spectroscopic studies of light-harvesting
complexes of photosynthetic bacteria. J. Schmidt
9:40 — 358. Energy transfer studies of single phycoerythrocyanin
molecules. P. Zehetmayer, H. Scheer, C. Bräuchle, A. Zumbusch
10:00 — Intermission.
10:20 — 359. Equilibrium fluctuations and energy transfer of reactions
and conformational dynamics of proteins. R. M. Hochstrasser, E. Mei, J.
Tang, M. Lee
11:00 — 360. Multichromophoric dendrimers: Model systems for the study
of energy transfer processes in antenna systems. F. C. De Schryver, J.
Hofkens
11:20 — 361. Hidden Markov models applied to time correlated single
photon trajectories of single molecules. D. S. Talaga
11:40 — 362. NSOM measurements of single human retinal lipofuscin
granules. J. D. Simon, L. E. Lamb, R. C. Dunn, J. R. Krogmeier, T.
Sarna, C. M. R. Clancy, N. M. Haralampus-Grynaviski, A. Pawlak, M. Rozanowska
Thursday afternoon (Chair, Rob
Dickson)
1:40 — 379. Single molecule studies of living colors and
transmembrane proteins on cells. M. F. Garcia-Parajo, B. I. de Bakker,
M. Koopman, C. Figdor, N. F. van Hulst
2:00 — 380. Phonon-assisted exciton delocalization in 1-D biological
antenna complexes. Z. G. Yu, X. Song.
Withdrawn.
2:20 — 381. Single molecule rotational mobility in glassy state
polymers. A. P. Bartko, R. M. Dickson
2:40 — Intermission.
3:00 — 382. Observation of single molecule diffusion in liquids near
interfaces. J. Schuster, F. Cichos, J. Wrachtrup, C. von Borczyskowski
3:20 — 383. Luminescence lifetimes of single molecules in disordered
media. T. Plakhotnik, E. A. Donley
3:40 — 384. Submicron structure of a Shpol'skii matrix: A
single-molecule study of dibenzanthanthrene in n-tetradecane. A. Bloeß, Y.
Durand, M. Matsushita, E. J. J. Groenen, J. Schmidt